Ex vivo metabolite profiling of paediatric central nervous system tumours reveals prognostic markers.

Bennett, Christopher D and Gill, Simrandip K and Kohe, Sarah E and Wilson, Martin P and Davies, Nigel P and Arvanitis, Theodoros N and Tennant, Daniel A and Peet, Andrew C (2019) Ex vivo metabolite profiling of paediatric central nervous system tumours reveals prognostic markers. Scientific reports, 9 (1). p. 10473. ISSN 2045-2322.

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Official URL: https://www.nature.com/articles/s41598-019-45900-x

Abstract

Brain tumours are the most common cause of cancer death in children. Molecular studies have greatly improved our understanding of these tumours but tumour metabolism is underexplored. Metabolites measured in vivo have been reported as prognostic biomarkers of these tumours but analysis of surgically resected tumour tissue allows a more extensive set of metabolites to be measured aiding biomarker discovery and providing validation of in vivo findings. In this study, metabolites were quantified across a range of paediatric brain tumours using H-High-Resolution Magic Angle Spinning nuclear magnetic resonance spectroscopy (HR-MAS) and their prognostic potential investigated. HR-MAS was performed on pre-treatment frozen tumour tissue from a single centre. Univariate and multivariate Cox regression was used to examine the ability of metabolites to predict survival. The models were cross validated using C-indices and further validated by splitting the cohort into two. Higher concentrations of glutamine were predictive of a longer overall survival, whilst higher concentrations of lipids were predictive of a shorter overall survival. These metabolites were predictive independent of diagnosis, as demonstrated in multivariate Cox regression models. Whilst accurate quantification of metabolites such as glutamine in vivo is challenging, metabolites show promise as prognostic markers due to development of optimised detection methods and increasing use of 3 T clinical scanners.

Item Type: Article
Subjects: WH Haemic and lymphatic systems. Haematology
WS Paediatrics. Child health
Divisions: Clinical Support > Pathology
Planned IP Care > Oncology and Clinical Haematology
Womens and Childrens > Paediatrics
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Depositing User: Mr Philip O'Reilly
Date Deposited: 29 Jul 2019 14:55
Last Modified: 29 Jul 2019 14:55
URI: http://www.repository.uhblibrary.co.uk/id/eprint/2263

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