Brief O uploading during continuous hypothermic machine perfusion is simple yet effective oxygenation method to improve initial kidney function in a porcine autotransplant model.

Darius, T and Vergauwen, M and Smith, T and Gerin, I and Joris, V and Mueller, M and Aydin, S and Muller, X and Schlegel, Andrea and Nath, J and Ludwig, C and Dessy, C and Many, M C and Bommer, G and Dutkowski, P and Gianello, P and Mourad, M (2020) Brief O uploading during continuous hypothermic machine perfusion is simple yet effective oxygenation method to improve initial kidney function in a porcine autotransplant model. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. ISSN 1600-6143. This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs

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Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/aj...

Abstract

With oxygenation proposed as a resuscitative measure during hypothermic models of preservation, the aim of this study was to evaluate the optimal start time of oxygenation during continuous hypothermic machine perfusion (HMP). In this porcine ischemia-reperfusion autotransplant model, the left kidney of a ±40 kg pig was exposed to 30 minutes of warm ischemia prior to 22 hours of HMP and autotransplantation. Kidneys were randomized to receive 2 hours of oxygenation during HMP either at the start (n=6), or end of the perfusion (n=5) and outcomes were compared to standard, non-oxygenated HMP (n=6) and continuous oxygenated HMP (n=8). The brief initial and continuous oxygenated HMP groups were associated with superior graft recovery compared to either standard, non-oxygenated HMP or kidneys oxygenated at the end of HMP. This correlated with significant metabolic differences in perfusate (e.g. lactate, succinate, flavin mononucleotide) and tissues (e.g. succinate, ATP, HIF-1α, Nrf2) suggesting superior mitochondrial preservation with initial oxygenation. Brief initial O uploading during HMP at procurement site might be an easy and effective preservation strategy to maintain aerobic metabolism, protect mitochondria and achieve an improved early renal graft function compared with standard HMP or oxygen supply shortly at the end of HMP preservation.

Item Type: Article
Additional Information: This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs
Subjects: WJ Urogenital system. Urology
Divisions: Emergency Services > Renal
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Depositing User: Mr Philip O'Reilly
Date Deposited: 07 Feb 2020 14:51
Last Modified: 07 Feb 2020 14:51
URI: http://www.repository.uhblibrary.co.uk/id/eprint/2839

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