Intrahepatic macrophage populations in the pathophysiology of primary sclerosing cholangitis.

Chen, Yung-Yi, Arndtz, Kathryn, Webb, Gwilym, Corrigan, Margaret, Akiror, Sarah, Liaskou, Evaggelia, Woodward, Paul, Adams, David H, Weston, Chris J and Hirschfield, Gideon M (2019) Intrahepatic macrophage populations in the pathophysiology of primary sclerosing cholangitis. JHEP reports (Online), 1 (5). pp. 369-376. ISSN 2589-5559.

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Background & Aims

Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by progressive inflammatory and fibrotic injury to the biliary tree. We sought to further delineate the contribution of macrophage lineages in PSC pathobiology.


Human liver tissues and/or blood samples from patients with PSC, primary biliary cholangitis, other non-cholestatic/non-autoimmune diseases, including alcohol-related liver disease and non-alcoholic steatohepatitis, as well as normal liver, were sourced from our liver transplantation program. Liver fibrosis was studied using Van Gieson staining, while the frequencies of infiltrating monocyte and macrophage lineages, both in the circulation and the liver, were investigated by flow cytometry, including the expression of TGR-5, a G protein-coupled receptor (GPBAR1/TGR-5).


Significantly higher frequencies of CD68CD206 macrophages were detected in the livers of patients with PSC (median 19.17%; IQR 7.25-32.8%; n = 15) compared to those of patients with other liver diseases (median 12.05%; IQR 5.61-16.03%; n = 12; 0.0373). CD16 monocytes, including both intermediate (CD14CD16) and non-classical (CD14CD16) monocytes, were preferentially recruited into chronically diseased livers, with the highest recruitment ratios in PSC (median 15.83%; IQR 9.66-29.5%; n = 15), compared to other liver diseases (median 6.66%; IQR 2.88-11.64%, n = 14, 0.0152). The expression of TGR-5 on CD68 intrahepatic macrophages was increased in chronic liver disease; TGR-5 expression on intrahepatic macrophages was highest in PSC (median 36.32%; IQR 17.71-63.61%; n = 6) and most TGR-5 macrophages were CD68CD206 macrophages.


Underlying a potential role for macrophages in PSC pathobiology, we demonstrate, using patient-derived tissue, increased CD16 monocyte recruitment and a higher frequency of CD68CD206 macrophages in the livers of patients with PSC; the CD68CD206 macrophage subset was associated with significantly higher TGR-5 expression in PSC.

Lay summary

Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease associated with progressive inflammation of the bile duct, leading to fibrosis and end-stage liver disease. In this study we explore the role of a type of immune cell, the macrophage, in contributing to PSC as a disease, hoping that our findings direct scientists towards new treatment targets. Our findings based on human liver and blood analyses demonstrate a greater frequency of a particular subset of immune cell, the CD68CD206 macrophage, with significantly higher TGR-5 expression on this subset in PSC.

Item Type: Article
Subjects: WI Digestive system. Gastroenterology
WK Endocrine system. Endocrinology
Divisions: Planned IP Care > Gastroentrology
Related URLs:
Depositing User: Mr Muneeb Liaquat
Date Deposited: 14 Feb 2020 14:15
Last Modified: 14 Feb 2020 14:15

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