Paneth cell alterations during ischemia-reperfusion, follow-up, and graft rejection after intestinal transplantation.

Kip, Anna M, Ceulemans, Laurens J, Hundscheid, Inca Hr, Canovai, Emilio, Hartog, Hermien, Brown, Rachel M, Corcos, Olivier, Joly, Francisca, De Hertogh, Gert, Gupte, Girish, Dejong, Cornelis Hc, Olde Damink, Steven Wm, Pirenne, Jacques, Mirza, Darius F and Lenaerts, Kaatje (2020) Paneth cell alterations during ischemia-reperfusion, follow-up, and graft rejection after intestinal transplantation. Transplantation. ISSN 1534-6080. This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs

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Official URL: https://journals.lww.com/transplantjournal/Abstrac...

Abstract

BACKGROUND

Ischemia-reperfusion (IR) injury is inevitable during intestinal transplantation (ITx) and executes a key role in the evolution towards rejection. Paneth cells (PC) are crucial for epithelial immune defense and highly vulnerable to IR injury. We investigated the effect of ITx on PC after reperfusion (T0), during follow-up, and rejection. Moreover, we investigated whether PC loss was associated with impaired graft homeostasis.

METHODS

Endoscopic biopsies, collected according to center-protocol and at rejection episodes, were retrospectively included (n=28 ITx, n=119 biopsies) Biopsies were immunohistochemically co-stained for PC (lysozyme) and apoptosis, and PC/crypt and lysozyme intensity were scored.

RESULTS

We observed a decrease in PC/crypt and lysozyme intensity in the first week after ITx (W1) compared to T0. There was a tendency towards a larger decline in PC/crypt (p=0.08) and lysozyme intensity (p=0.08) in W1 in patients who later developed rejection compared to patients without rejection. Follow-up biopsies showed that the PC number recovered, whereas lysozyme intensity remained reduced. This persisting innate immune defect may contribute to the well-known vulnerability of the intestine to infection. There was no clear evidence that PC were affected throughout rejection.

CONCLUSION

This study revealed a transient fall in PC numbers in the early post-ITx period, but a permanent reduction in lysozyme intensity following ITx. Further research is needed to determine the potential clinical impact of PC impairment after ITx.

Item Type: Article
Additional Information: This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs
Subjects: WI Digestive system. Gastroenterology
WO Surgery
Divisions: Planned IP Care > Gastroentrology
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Depositing User: Mrs Yolande Brookes
Date Deposited: 10 Apr 2020 15:33
Last Modified: 10 Apr 2020 15:33
URI: http://www.repository.uhblibrary.co.uk/id/eprint/2985

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