The Effect of β-Adrenoceptor Agonists on Leucocyte-Endothelial Adhesion in a Rodent Model of Laparotomy and Endotoxemia.

Bangash, Mansoor Nawaz, Abbott, Tom E F, Patel, Nimesh S A, Hinds, Charles Johnston, Thiemermann, Christoph and Pearse, Rupert Mark (2020) The Effect of β-Adrenoceptor Agonists on Leucocyte-Endothelial Adhesion in a Rodent Model of Laparotomy and Endotoxemia. Frontiers in immunology, 11. p. 1001. ISSN 1664-3224.

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Official URL: https://www.frontiersin.org/articles/10.3389/fimmu...

Abstract

The β-adrenoceptor agonist dopexamine may possess anti-inflammatory actions which could reduce organ injury during endotoxemia and laparotomy. Related effects on leucocyte-endothelial adhesion remain unclear. Thirty anesthetized Wistar rats underwent laparotomy followed by induction of endotoxemia with lipopolysaccharide and peptidoglycan ( = 24) or sham ( = 6). Animals received dopexamine at 0.5 or 1 μg kg min (D0.5 and D1), salbutamol at 0.1 μg kg min, or saline vehicle (Sham and Control) for 5 h. Intravital microscopy was performed in the ileum of the small intestine to assess leucocyteendothelial adhesion, arteriolar diameter, and functional capillary density. Global hemodynamics and biochemical indices of renal and hepatic function were also measured. Endotoxemia was associated with an increase in adherent leucocytes in post-capillary venules, intestinal arteriolar vasoconstriction as well-reduced arterial pressure and relative cardiac index, but functional capillary density in the muscularis was not significantly altered. Dopexamine and salbutamol administration were associated with reduced leucocyte-endothelial adhesion in post-capillary venules compared to control animals. Arteriolar diameter, arterial pressure and relative cardiac index all remained similar between treated animals and controls. Functional capillary density was similar for all groups. Control group creatinine was significantly increased compared to sham and higher dose dopexamine. In a rodent model of laparotomy and endotoxemia, β-agonists were associated with reduced leucocyte-endothelial adhesion in post-capillary venules. This effect may explain some of the anti-inflammatory actions of these agents.

Item Type: Article
Subjects: QV Pharmacology
WI Digestive system. Gastroenterology
WO Surgery
Divisions: Clinical Support > Anaesthetics
Clinical Support > Critical Care
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Depositing User: Mrs Yolande Brookes
Date Deposited: 23 Jul 2020 12:15
Last Modified: 23 Jul 2020 12:15
URI: http://www.repository.uhblibrary.co.uk/id/eprint/3292

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