Ultrastructure of Cell Trafficking Pathways and Coronavirus: How to Recognise the Wolf Amongst the Sheep.

Neil, Desley, Moran, Linda, Horsfield, Catherine, Curtis, Elizabeth, Swann, Olivia, Barclay, Wendy, Hanley, Brian, Hollinshead, Michael and Roufosse, Candice (2020) Ultrastructure of Cell Trafficking Pathways and Coronavirus: How to Recognise the Wolf Amongst the Sheep. The Journal of pathology. ISSN 1096-9896.

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Official URL: https://onlinelibrary.wiley.com/doi/10.1002/path.5...

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) pandemic has resulted in an urgent need to understand the pathophysiology of SARS-CoV2 infection, to assist in the identification of treatment strategies. Viral tissue tropism is an active area of investigation, one approach to which is identification of virus within tissues by electron microscopy of post-mortem and surgical specimens. Most diagnostic histopathologists have limited understanding of the ultrastructural features of normal cell trafficking pathways, which can resemble intra- and extracellular coronavirus; in addition, viral replication pathways make use of these trafficking pathways. Herein, we review these pathways and their ultrastructural appearances, with emphasis on structures which may be confused with coronavirus. In particular, we draw attention to the fact that, when using routine fixation and processing, the typical 'crown' that characterises a coronavirus is not readily identified on intracellular virions, which are located in membrane-bound vacuoles. In addition, the viral nucleocapsid is seen as black dots within the virion and is more discriminatory in differentiating virions from other cellular structures. The identification of the viral replication organelle, a collection of membranous structures (convoluted membranes) seen at a relatively low scanning power, may help draw attention to infected cells, which can be sparse. This article is protected by copyright. All rights reserved.

Item Type: Article
Subjects: QW Microbiology. Immunology
QY Clinical pathology
WC Communicabable diseases
Divisions: Clinical Support > Infectious Diseases
Clinical Support > Pathology
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Depositing User: Mr Philip O'Reilly
Date Deposited: 23 Sep 2020 12:31
Last Modified: 23 Sep 2020 12:31
URI: http://www.repository.uhblibrary.co.uk/id/eprint/3464

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