Transmission, adaptation and geographical spread of the Liverpool epidemic strain.

Moore, Matthew P, Lamont, Iain L, Williams, David, Paterson, Steve, Kukavica-Ibrulj, Irena, Tucker, Nicholas P, Kenna, Dervla T D, Turton, Jane F, Jeukens, Julie, Freschi, Luca, Wee, Bryan A, Loman, Nicholas J, Holden, Stephen, Manzoor, Susan, Hawkey, Peter M, Southern, Kevin W, Walshaw, Martin J, Levesque, Roger C, Fothergill, Joanne L and Winstanley, Craig (2021) Transmission, adaptation and geographical spread of the Liverpool epidemic strain. Microbial genomics. ISSN 2057-5858. This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs

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Abstract

The Liverpool epidemic strain (LES) is an important transmissible clonal lineage of that chronically infects the lungs of people with cystic fibrosis (CF). Previous studies have focused on the genomics of the LES in a limited number of isolates, mostly from one CF centre in the UK, and from studies highlighting identification of the LES in Canada. Here we significantly extend the current LES genome database by genome sequencing 91 isolates from multiple CF centres across the UK, and we describe the comparative genomics of this large collection of LES isolates from the UK and Canada. Phylogenetic analysis revealed that the 145 LES genomes analysed formed a distinct clonal lineage when compared with the wider population. Notably, the isolates formed two clades: one associated with isolates from Canada, and the other associated with UK isolates. Further analysis of the UK LES isolates revealed clustering by clinic geography. Where isolates clustered closely together, the association was often supported by clinical data linking isolates or patients. When compared with the earliest known isolate, LESB58 (from 1988), many UK LES isolates shared common loss-of-function mutations, such as in genes and . Other loss-of-function mutations identified in previous studies as common adaptations during CF chronic lung infections were also identified in multiple LES isolates. Analysis of the LES accessory genome (including genomic islands and prophages) revealed variations in the carriage of large genomic regions, with some evidence for shared genomic island/prophage complement according to clinic location. Our study reveals divergence and adaptation during the spread of the LES, within the UK and between continents.

Item Type: Article
Additional Information: This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs
Subjects: QU Biochemistry
QV Pharmacology
QW Microbiology. Immunology
WC Communicabable diseases
Divisions: Clinical Support > Infection Control
Related URLs:
Depositing User: Jamie Edgar
Date Deposited: 25 Mar 2021 15:39
Last Modified: 25 Mar 2021 15:39
URI: http://www.repository.uhblibrary.co.uk/id/eprint/4129

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