Molecular Characterisation of Plasmids Encoding bla from Faecal Escherichia coli in Travellers Returning to the UK from South Asia.

Bevan, E R, Powell, M J, Toleman, M A, Thomas, C M, Piddock, L J V and Hawkey, P M (2021) Molecular Characterisation of Plasmids Encoding bla from Faecal Escherichia coli in Travellers Returning to the UK from South Asia. The Journal of hospital infection. ISSN 1532-2939. This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs

Full text not available from this repository.
Official URL: http://www.journals.elsevier.com/journal-of-hospit...

Abstract

BACKGROUND

The global prevalence of ESBL-producing Escherichia coli is rising and is dominated by bla spread by plasmids. Travellers to South Asia from Western Europe have high rates of acquisition of faecal CTX-M-producing E. coli (CTX-M-EC).

AIMS

We aimed to determine the conjugative ability of CTX-M-EC acquired by healthy volunteers after travel to South Asia, the proportion of travel-acquired CTX-M-EC where bla is encoded on a plasmid versus on the bacterial chromosome, and the relatedness of travel-acquired CTX-M-EC plasmids to previously sequenced plasmids.

METHODS

Faecal samples were collected pre- and post-travel from 23 volunteers who visited South Asia, and the CTX-M-EC were cultured. After short- and long-read sequencing, ten plasmid sequences were identified and compared to previously sequenced plasmids in Genbank. Conjugation to E. coli K-12 was undertaken using filter mating.

FINDINGS

35% of CTX-M-EC isolates tested transferred the bla plasmid by conjugation. Travel-acquired CTX-M-EC carried bla on a plasmid in 62% of isolates, whereas 38% of isolates had bla on the chromosome. CTX-M-EC plasmids acquired after travel to South Asia have close homology to previously described epidemic plasmids which are widely disseminated in humans, animals, and the natural environment.

CONCLUSION

Globally successful epidemic plasmids are involved in the spread of CTX-M-EC. Targeted strategies may be used to displace such plasmids from the host strain as part of efforts in infection prevention and control in healthcare settings. Bacteria with bla plasmids were readily acquired by healthy volunteers and were carried on return to the UK, providing opportunities for onward dissemination.

Item Type: Article
Additional Information: This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs
Subjects: QW Microbiology. Immunology
Divisions: Clinical Support > Infectious Diseases
Related URLs:
Depositing User: Jamie Edgar
Date Deposited: 23 Apr 2021 14:50
Last Modified: 23 Apr 2021 14:50
URI: http://www.repository.uhblibrary.co.uk/id/eprint/4246

Actions (login required)

View Item View Item